Identification of N-linked carbohydrates from severe acute respiratory syndrome (SARS) spike glycoprotein.
Identifieur interne : 002529 ( Main/Exploration ); précédent : 002528; suivant : 002530Identification of N-linked carbohydrates from severe acute respiratory syndrome (SARS) spike glycoprotein.
Auteurs : Gayle Ritchie [Royaume-Uni] ; David J. Harvey ; Friederike Feldmann ; Ute Stroeher ; Heinz Feldmann ; Louise Royle ; Raymond A. Dwek ; Pauline M. RuddSource :
- Virology [ 1096-0341 ] ; 2010.
Descripteurs français
- KwdFr :
- Animaux, Cellules Vero, Chromatographie en phase liquide à haute performance, Glucides (), Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (), Glycosylation, Polyosides (), Protéines de l'enveloppe virale (), Spectrométrie de masse ESI, Spectrométrie de masse MALDI, Virus du SRAS ().
- MESH :
English descriptors
- KwdEn :
- Animals, Carbohydrates (chemistry), Chlorocebus aethiops, Chromatography, High Pressure Liquid, Glycosylation, Membrane Glycoproteins (chemistry), Polysaccharides (chemistry), SARS Virus (chemistry), Spectrometry, Mass, Electrospray Ionization, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Spike Glycoprotein, Coronavirus, Vero Cells, Viral Envelope Proteins (chemistry).
- MESH :
- chemical , chemistry : Carbohydrates, Membrane Glycoproteins, Polysaccharides, Viral Envelope Proteins.
- chemistry : SARS Virus.
- Animals, Chlorocebus aethiops, Chromatography, High Pressure Liquid, Glycosylation, Spectrometry, Mass, Electrospray Ionization, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Spike Glycoprotein, Coronavirus, Vero Cells.
Abstract
N-glycans were released from the SARS coronavirus (SARS-CoV) spike glycoprotein produced in Vero E6 cells and their structures were determined by a combination of matrix-assisted laser desorption/ionization (MALDI) mass spectrometry, negative ion electrospray collision-induced dissociation time-of-flight mass spectrometry and normal-phase high-performance liquid chromatography with exoglycosidase digestion. Major glycans were high-mannose (Man(5-9)GlcNAc(2)), hybrid and bi-, tri- and tetra-antennary complex with and without bisecting GlcNAc and core fucose. Complex glycans with fewer than the full complement of galactose residues were present and sialylation was negligible. Treatment with the glucosidase inhibitor N-butyl-deoxynojirimycin (NB-DNJ) inhibited N-glycan processing as evidenced by the appearance of glycans of composition Glc(3)Man(7-9)GlcNAc(2). However, some complex glycans remained suggesting the presence of an alpha-endomannosidase. Our data in tissue culture indicate that inhibition of N-glycan processing may be considered as a therapeutic strategy against SARS CoV infections.
DOI: 10.1016/j.virol.2009.12.020
PubMed: 20129637
Affiliations:
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Le document en format XML
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Harvey</name></author><author><name sortKey="Feldmann, Friederike" sort="Feldmann, Friederike" uniqKey="Feldmann F" first="Friederike" last="Feldmann">Friederike Feldmann</name></author><author><name sortKey="Stroeher, Ute" sort="Stroeher, Ute" uniqKey="Stroeher U" first="Ute" last="Stroeher">Ute Stroeher</name></author><author><name sortKey="Feldmann, Heinz" sort="Feldmann, Heinz" uniqKey="Feldmann H" first="Heinz" last="Feldmann">Heinz Feldmann</name></author><author><name sortKey="Royle, Louise" sort="Royle, Louise" uniqKey="Royle L" first="Louise" last="Royle">Louise Royle</name></author><author><name sortKey="Dwek, Raymond A" sort="Dwek, Raymond A" uniqKey="Dwek R" first="Raymond A" last="Dwek">Raymond A. Dwek</name></author><author><name sortKey="Rudd, Pauline M" sort="Rudd, Pauline M" uniqKey="Rudd P" first="Pauline M" last="Rudd">Pauline M. 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Major glycans were high-mannose (Man(5-9)GlcNAc(2)), hybrid and bi-, tri- and tetra-antennary complex with and without bisecting GlcNAc and core fucose. Complex glycans with fewer than the full complement of galactose residues were present and sialylation was negligible. Treatment with the glucosidase inhibitor N-butyl-deoxynojirimycin (NB-DNJ) inhibited N-glycan processing as evidenced by the appearance of glycans of composition Glc(3)Man(7-9)GlcNAc(2). However, some complex glycans remained suggesting the presence of an alpha-endomannosidase. Our data in tissue culture indicate that inhibition of N-glycan processing may be considered as a therapeutic strategy against SARS CoV infections.</div></front></TEI><affiliations><list><country><li>Royaume-Uni</li></country></list><tree><noCountry><name sortKey="Dwek, Raymond A" sort="Dwek, Raymond A" uniqKey="Dwek R" first="Raymond A" last="Dwek">Raymond A. Dwek</name><name sortKey="Feldmann, Friederike" sort="Feldmann, Friederike" uniqKey="Feldmann F" first="Friederike" last="Feldmann">Friederike Feldmann</name><name sortKey="Feldmann, Heinz" sort="Feldmann, Heinz" uniqKey="Feldmann H" first="Heinz" last="Feldmann">Heinz Feldmann</name><name sortKey="Harvey, David J" sort="Harvey, David J" uniqKey="Harvey D" first="David J" last="Harvey">David J. Harvey</name><name sortKey="Royle, Louise" sort="Royle, Louise" uniqKey="Royle L" first="Louise" last="Royle">Louise Royle</name><name sortKey="Rudd, Pauline M" sort="Rudd, Pauline M" uniqKey="Rudd P" first="Pauline M" last="Rudd">Pauline M. Rudd</name><name sortKey="Stroeher, Ute" sort="Stroeher, Ute" uniqKey="Stroeher U" first="Ute" last="Stroeher">Ute Stroeher</name></noCountry><country name="Royaume-Uni"><noRegion><name sortKey="Ritchie, Gayle" sort="Ritchie, Gayle" uniqKey="Ritchie G" first="Gayle" last="Ritchie">Gayle Ritchie</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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